UNIVERSIDAD DE GUAYAQUIL

DEPARTMENT OF CHEMICAL SCIENCES

Ciudadela Universitaria ¨Dr. Salvador Allende¨

Telephone: 2293680, E-mail: fcquimic@ug.edu.ec

Guayaquil, Ecuador

FINAL REPORT - SUMMARY

CODE: 35/05

TITLE:

Determination of the possible Hepatoprotector potential of the product Burbur Detox originating from NutraMedix LLC Laboratories, Jupiter, Florida, United States.

OBJETIVES:

Study the possible Hepatoprotector effect of the medication known as Burbur Detox, measured by the variations experienced by the Pyruvate and Oxaloacetic transaminase enzymes when a hepatotoxic agent is administered to laboratory animals.

Description OF THE DOSAGE, METHOD of administration and duration of the test:

The administered volume of acetaminophen and N-Acetyl-cysteine was 10 mL/kg of body weight, and 1.5 mL/kg for Burbur Detox.

Medication was administered orally through an intragastric canula. 

The dosage of acetaminophen and N-Acetyl-cysteine was 600 mg/kg.

AnalIticAL Results:

In the table one may observe the mean and standard deviation for the four test groups, as well as the statistical comparison of the results of the aforementioned procedures. 

Table # 2. DETERMINATION OF THE ENZYMES GPT Y GOT (U/L)
GROUP	Values of the Glutamate Pyruvate Transaminase (GPT) and Glutamic-Oxaloacetic Transaminase (GOT) (U/L)
	GOT		GPT
	0 HOURS	24 HOURS	0 HOURS	24 HOURS
I. Control	154.0 ± 18.4 a	151 ± 14.7 b	42.2 ± 3.6 d	44.4 ± 4.7 e
II. Acetaminophen	149.4 ± 12.5 a	319.0 ± 17.0 c	48.2 ± 12.44 d	369.0 ± 77.18 f
III. Positive Control	146.2 ± 16.1 a	151.2 ± 21.6 b	54.0 ± 14.9 d	51.6 ± 7.4 e
IV. IV. Burbur Detox	176.6 ± 34.8 a	200.5 ± 85.5 b	54.8 ± 6.8 d	89.5 ± 48.8 e

a, b, c, d: Statistical significance (p<0.05).

As can be appreciated from the table, three groups did not differ from each other: Burbur, N-Acetyl-cysteine, and those that received no treatment.  This in spite of the fact that, as may be observed, the values obtained from the Burbur group were high.  However, they are below Acetaminophen, which is logical if one takes into account that a product (Acetaminophen) that causes liver damage is being administered to the animals.  No matter how much protection there is, there will always be damage, reflected in elevated transaminases.  But it does differ from the positive control that received N-Acetyl-cysteine, that is, the antidote to Acetaminophen.

Conclusions:

Burbur Detox was capable of protecting the liver when acetaminophen, a hepatoxic agent, was administered, in specific concentrations, in laboratory tests using mice.

GENERAL ConclusioNS:

Burbur Detox was demonstrated to possess a HEPATO-PROTECTOR effect at the indicated dosage in the established model for this type of study.

Date: 05/27/05