UNIVERSIDAD DE GUAYAQUIL

DEPARTMENT OF CHEMICAL SCIENCES

Ciudadela Universitaria ¨Dr. Salvador Allende¨

Telephone: 2293680, E-mail: fcquimic@ug.edu.ec

Guayaquil, Ecuador

FINAL REPORT - SUMMARY

 

 

CODE: 28/05

 

TITLE:

Determination of the possible weight loss effect of the product known as Burbur Detox, originating from NutraMedix Laboratories, LLC. Florida, United States.

 

 

OBJECTIVES:

To study the effectiveness of Burbur Detox in producing effects on weight gain in laboratory mice, following techniques described in the literature.

 

 

DOSAGE USED IN THE TEST:

In this study 0.5 mL/200 g of animal body weight was utilized, in a daily administration.  The product was dissolved in water at a rate of 40 drops per 80 mL of water.

 

 

principal TEST:

 

 

METHODS AND TECHNIQUES:

 

Study Material: Burbur Detox

 

Animal Model: A single rodent species (mouse) was utilized, with a minimum of 5 animals of a single sex in each group.  In this case, male mice with an average weight within ± 20% (3), belonging to the Wister line and coming from the Chemistry Department of the University of Guayaquil were appropriate and were utilized in the experiment. 

 

The animals were maintained in quarantine conditions and were acclimated according to established procedures (4,5), said period having a duration of five days minimum.

 

Access to the water was "ad libitum”, but food was given in a controlled form, a quantity measured by noting what was consumed and what was left the next day. (6,7)

 

The animals were randomly distributed from within the different groups.(8)

 

Food was denied 4 hours before exposure to the test material.

 

The experiment lasted 12 days (5 of acclimation and 7 of test).

 

Each mouse was given the stipulated quantity of food appropriate for the species.

 

At the end of the experiment all the animals were euthanized, following the procedures of Refinement, to avoid pain and suffering by the animals.

 

 

 

From the graph one can appreciate how those animals in the control group gained weight as compared to those who received treatment.

 

 

CONCLUSIONS:

Burbur Detox had a weight loss effect on laboratory rodents during the week of the experiment.

 

 

Date: 08/05/05